Abstract:

Researchers have confirmed that canine distemper virus is the cause of an epidemic that killed over 1/3 of the lion population of Serengeti. Genetic analysis indicates that the organism is a new variant that probably mutated from the form that infects domestic dogs.

Full Text:

“If you were a dog, I’d say you had canine distemper,” veterinarian Melody Roelke-Parker remembers thinking in February 1994 as she watched a male lion in Tanzania convulse and twitch. “But you’re a cat, and cats don’t get canine distemper.” Yet by June of that year, Roelke-Parker–who works at Tanzania’s Serengeti Wildlife Research Institute–had her suspicions confirmed: Researchers had identified canine distemper virus (CDV) as the culprit in an epidemic that wiped out more than a third of the Serengeti’s lion population. The pathogen, a member of the morbillivirus family, also killed hyenas, leopards, and bat-eared foxes (Science, 17 June 1994, p. 1664). But how a virus historically restricted to dogs suddenly jumped to cats has remained a puzzle.

Now, thanks to a new genetic analysis, an answer is beginning to appear: The Serengeti organism seems to be a new variant, or biotype. In this week’s issue of Nature, Linda Munson, a veterinary pathologist at the University of Tennessee’s College of Veterinary Medicine, and her colleagues report that the Serengeti strain is genetically different from normal CDV. While the researchers have not yet been able to show how the genetic shift caused the new infection pattern, they have been able to trace environmental changes that apparently prompted the mutation: growing human settlements along the Serengeti National Park’s western border, with large populations of CDV-infected domestic dogs. “When you have a wildlife population in close proximity to domestic animals like this, you’re going to see an exchange of diseases–and you’re going to encourage the emergence of successful mutations,” Munson says.

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This, to other researchers, is convincing evidence. “These findings fit with the overall pattern of emerging viruses” such as that of the hantavirus, says Richard J. Montali, head of pathology at the Smithsonian Institution’s National Zoological Park in Washington, D.C. “This is one of the most globally important cases,” he adds, “because it points out that morbilliviruses have made incredible gains in evolving to increase their host range.” Although CDV itself has previously been shown to infect black-footed ferrets, new morbilli-viruses have recently been identified in seals, dolphins, and horses. Further analysis of the Serengeti variant might reveal genetic changes that make such expansion possible.

Munson and her team suspected they were looking at something new when they learned that the pathology of the disease had changed in the jump to the cats. “It infects the lions’ hippocampus, whereas the other strain primarily causes inflammation of the brain stem in dogs,” explains Munson. And while both strains cause pneumonia, they do so in different ways: Normally CDV affects dogs’ bronchial tubes, but the Serengeti variant attacks the alveoli, air sacs in the lungs. These changes could simply be the result of new opportunities in a new host, Munson says. “But when you see a virus that not only has a broader species range but affects different tissues, you suspect you’re looking at an emerging biotype,” she adds.

To confirm these suspicions, her team compared virus samples from the lions to the best known strain, Onderstepoort, which was isolated from a domestic dog in South Africa. Margaret Carpenter, a molecular biologist at the U.S. National Cancer Institute’s research center in Frederick, Maryland, who did the genetic analysis, explains that the group focused on a well-studied gene, coding for a phosphoprotein that helps transcribe the viral genome as it prepares to replicate. In her analysis of a 389-base-pair fragment, Carpenter found 18 nucleotide substitutions, suggesting that the two viral strains were “significantly different.”

Changes in genome replication could underlie the virus’s ability to jump into a new host, says Carpenter. Then again, she says, “we really don’t know which parts of the virus’s genome need to be changed in order for it to switch hosts.” Carpenter is now sequencing part of the viral hemagglutinin gene, which is thought to affect the host’s immune response. Again, mutations in this gene might enable the virus to take up a new residence.

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Although the team has not been able to pinpoint the genetic mechanism for the jump, they have been able to point a finger at the jumping point. Between 1993 and 1994, a CDV epidemic swept through villages to the west of the Serengeti, killing thousands of domestic dogs. Monoclonal antibody tests show similarities between this strain and the one that infected the lions. The researchers propose that the virus then entered the park, perhaps via jackals and spotted hyenas, which frequently scavenge near humans. Because CDV is shed in mucus, these animals, in turn, probably infected lions at kill sites, where there is often a lot of biting and snarling between species.

Then, between February and October 1994, at least 1000 of the park’s 3000 lions are thought to have died of the disease; the survivors probably developed immunity. Like all morbilliviruses, CDV requires a susceptible population to sustain itself, and no new cases have been seen in the last year.

The lion population is now on its way to recovery, reports Craig Packer, director of the Serengeti Lion Project and one of the study’s authors. Veterinarians have also launched a program to vaccinate the local domestic dogs against CDV and other diseases–a step that they hope will stop the virus before it jumps again.

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